Q. 46. The explant used in tissue culture must show
(A) Encystation
(B) Sporulation
(C) Dioecy
(D) Totipotency
Answer:
(D) Totipotency
Explanation:
According to the passage, plant tissue culture relies entirely on “totipotency,” which is the special inherent property of a single plant cell to generate an entire whole plant.
Q. 47. Virus free plants of banana are developed by using
(A) Protoplasts
(B) Meristem
(C) Cotyledon
(D) Leaf
Answer:
(B) Meristem
Explanation:
The passage specifies that the apical or axillary meristem is free of virus and can be removed and cultured in-vitro to obtain virus-free plants of banana, sugarcane, and potato.
Q. 48. Pomato is developed by ________ technique.
(A) Micropropagation
(B) Mutation breeding
(C) Biofortification
(D) Somatic hybridization
Answer:
(D) Somatic hybridization
Explanation:
The passage highlights that Pomato is a new hybrid plant formed by fusing the protoplast of a tomato with that of a potato, a distinct process known as somatic hybridization.
Q. 49. In tissue culture, the nutrient medium usually contains ________ as a carbon source.
(A) Sucrose
(B) Maltose
(C) Carbon dioxide
(D) Calcium carbonate
Answer:
(A) Sucrose
Explanation:
The text explicitly mentions that the standard nutrient medium used in tissue culture provides a carbon source “such as sucrose.”
Q. 50. Protoplasts are obtained by digestion of ________ of the cells.
(A) Nuclei
(B) Plasma membranes
(C) cell walls
(D) Proteins
Answer:
(C) cell walls
Explanation:
The passage notes that scientists can isolate single cells from plants and deliberately digest their “cell walls” to isolate the naked protoplasts within.
